Abstract
Molecular methods aimed at Yersinia pestis detection and identification mainly rely on the polymerase chain reaction (PCR) technique, including conventional PCR and real-time PCR. PCR-based assays are more sensitive and rapid than traditional methods, as the isolation and growth of Y. pestis from specimens are laborious and time-consuming. Y. pestis target gene sequences can also be quantified using real-time PCR. In this chapter, methods to extract Y. pestis genomic DNA from simulated soil samples are described. PCR and real-time PCR methods for sensitive and quantitative detection of Y. pestis are also discussed.
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Zhao, Y. (2018). PCR for Detection and Identification of Yersinia pestis . In: Yang, R. (eds) Yersinia Pestis Protocols. Springer Protocols Handbooks. Springer, Singapore. https://doi.org/10.1007/978-981-10-7947-4_28
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DOI: https://doi.org/10.1007/978-981-10-7947-4_28
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Publisher Name: Springer, Singapore
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Online ISBN: 978-981-10-7947-4
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