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The Replicative Detargeting FISH (ReD-FISH) Technique in Studies of Telomere Replication

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Fluorescence In Situ Hybridization (FISH)

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Abstract

Based on the chromosome orientation-FISH (CO-FISH) procedure, the replicative detargeting FISH (ReD-FISH) was developed as a unique tool to study the replicative patterns of telomeres located on individual chromosomal arms. This method is also suited for examination of telomeres of species belonging to different classes of animals, for which well-proliferated cell cultures can be established and maintained. ReD-FISH is based on pulsed inclusion of labeled brominated nucleotides in replicating DNA, destruction of regions with brominated analogs, and standard FISH with single-strand-specific probe/probes.

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Acknowledgments

This work was supported by the budget project VI.53.1.4. of the Federal Research Center Institute of Cytology and Genetics of SB RAS.

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Authors and Affiliations

  1. The Federal Research Center Institute of Cytology and Genetics of SB RAS, av. Lavrentjeva 10, 630090, Novosibirsk, Russia

    Nikolay Rubtsov & Natalya Zhdanova

Authors
  1. Nikolay Rubtsov
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  2. Natalya Zhdanova
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Corresponding author

Correspondence to Nikolay Rubtsov .

Editor information

Editors and Affiliations

  1. Institut für Humangenetik, Jena, Germany

    Thomas Liehr

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Rubtsov, N., Zhdanova, N. (2017). The Replicative Detargeting FISH (ReD-FISH) Technique in Studies of Telomere Replication. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH). Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52959-1_16

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  • DOI: https://doi.org/10.1007/978-3-662-52959-1_16

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-52957-7

  • Online ISBN: 978-3-662-52959-1

  • eBook Packages: Springer Protocols

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