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Genetic Fingerprinting Techniques for Molecular Characterisation of Microbes

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Analyzing Microbes

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

DNA fingerprints are commonly generated for a genetic characterisation of microbial populations or communities. The respective techniques are based either on hybridisation or on polymerase chain reaction (PCR). We present an overview and detailed protocols of the most frequently DNA fingerprinting techniques currently used in microbial ecology, including isolation of respective target sequences, set-ups of PCR reactions, and ways of detecting markers for generating fingerprints.

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Author information

Authors and Affiliations

  1. Department of Phytomedicine, Geisenheim Research Centre, Geisenheim, Germany

    Annette Reineke

  2. Department of Botany, Andhra University, Visakhapatnam, India

    K. Uma Devi

Authors
  1. Annette Reineke
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  2. K. Uma Devi
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Editor information

Editors and Affiliations

  1. National Bureau of Agriculturally Import, Kusumaur, Maunath Bhanjan, 275101, Uttar Pradesh, India

    Dilip Kumar Arora

  2. , Dep. Life Sciences, National Institute of Technology, Rourkela, Orissa, India

    Surajit Das

  3. National Bureau of Agriculturally Import, Kusumaur, Maunath Bhanjan, 275 101, Uttar Pradesh, India

    Mesapogu Sukumar

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© 2013 Springer-Verlag Berlin Heidelberg

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Reineke, A., Devi, K.U. (2013). Genetic Fingerprinting Techniques for Molecular Characterisation of Microbes. In: Arora, D., Das, S., Sukumar, M. (eds) Analyzing Microbes. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-34410-7_4

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  • DOI: https://doi.org/10.1007/978-3-642-34410-7_4

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-34409-1

  • Online ISBN: 978-3-642-34410-7

  • eBook Packages: Springer Protocols

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