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Advanced Protocols for Animal Transgenesis pp 25–42Cite as

Global Resources: Including Gene Trapped ES Cell Clones - Is Your Gene Already Knocked Out?

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Abstract

The design of any new mouse genetic modification today should start with careful scrutiny of the resources that are already available, through the internet, for information relating to your gene of interest. International mouse consortia are constantly providing new genetically modified alleles of virtually any gene in the mouse genome. Therefore, unless a very specific knock-in allele is required, it is more than likely that the envisaged mutation has already been obtained somewhere and made available in the form of embryonic stem (ES) cell clones, live animals, or cryopreserved sperm or embryos. In this chapter, I will review the current (November 2010) global resources that are available through the internet, where the most updated information about any given mouse gene should be examined, before any new experiment is planned or conducted. The knowledge and adequate use of all these global resources should speed up the acquisition of knowledge in the fields of biology, biomedicine, and biotechnology, while avoiding the redundant use of animals for experimentation and optimizing the use of limited funding resources. In this chapter, I will try to respond to two basic questions: where is my mouse? and what is known about my gene?

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Abbreviations

CMMR:

Canadian mouse mutant repository

CREATE:

Coordination of resources for conditional expression of mutated mouse alleles

EBI:

European Bioinformatics Institute

EMAP:

Edinburgh Mouse Atlas Project

EMBL:

European Molecular Biology Laboratory

EMMA:

European Mouse Mutant Archive

EMPReSS:

European mouse phenotyping resource of standardized screens

ENSEMBL:

A joint project between EMBL – EBI and the Wellcome Trust Sanger Institute to develop a software system which produces and maintains automatic annotation on selected eukaryotic genomes

ES:

Embryonic stem

ESPCR:

European Society of Pigment Cell Research

EUCOMM:

European Conditional Mouse Mutagenesis

EuMMCR:

European mouse mutant cell repository

EUMODIC:

European Mouse Disease Clinic

EUMORPHIA:

European Union Mouse Research for Public Health and Industrial Applications

EUROPHENOME:

Open source project to develop a software system for capturing, storing, and analyzing raw phenotyping data from SOPs contained in EMPReSS

FP6:

Framework Programme 6

ICS:

Institut Clinique de la Souris

IGTC:

International Gene Trap Consortium

IKMC:

International KnockOut Mouse Consortium

IMSR:

International Mouse Strain Resource

ISTT:

International Society for Transgenic Technologies

JAX:

The Jackson Laboratory

KOMP:

Knock-Out Mouse Project

KORC:

Knock-Out Rat Consortium

MGI:

Mouse Genome Informatics

MMRRC:

Mouse Mutant Regional Resource Centres

NBRP:

National BioResource Project for the Rat

NCBI:

National Center for Biotechnology Information

NIH:

National Institutes of Health

NorCOMM:

North-American Conditional Mouse Mutagenesis

OMIM:

Online Mendelian Inheritance in Man

RGD:

Rat genome database

RRRC:

Rat Resource & Research Centre

SNP:

Single nucleotide polymorphism

SOP:

Standard operating procedures

TIGM:

Texas A&M Institute for Genomic Medicine

UCSC:

University of California, Santa Cruz

ZFIN:

Zebrafish model organism database

ZGC:

Zebrafish gene collection

ZIRC:

Zebrafish International Resource Center

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Authors and Affiliations

  1. Department of Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB), Consejo Superior de Investigaciones Científicas (CSIC), Campus de Cantoblanco, C/Darwin 3, 28049, Madrid, Spain

    Lluis Montoliu

  2. Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), ISCIII, Madrid, Spain

    Lluis Montoliu

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  1. Lluis Montoliu
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Correspondence to Lluis Montoliu .

Editor information

Editors and Affiliations

  1. Div. Biology, California Institute of Technology, Pasadena, 91125, California, USA

    Shirley Pease

  2. Transgenic Animal Model Core, University of Michigan, Ann Arbor, 48109-5674, Michigan, USA

    Thomas L. Saunders

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Montoliu, L. (2011). Global Resources: Including Gene Trapped ES Cell Clones - Is Your Gene Already Knocked Out?. In: Pease, S., Saunders, T. (eds) Advanced Protocols for Animal Transgenesis. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20792-1_2

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  • DOI: https://doi.org/10.1007/978-3-642-20792-1_2

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-20791-4

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