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Rat Spermatogonial Stem Cell-Mediated Gene Transfer

  • Karen M. Chapman
  • Dalia Saidley-Alsaadi
  • Andrew E. Syvyk
  • James R. Shirley
  • Lindsay M. Thompson
  • F. Kent HamraEmail author
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

More than 20 years have passed since the advent of genetic manipulation of the mouse germline using cultures of pluripotent embryonic stem cells. Still, despite remarkable successes in the mouse, the application of stem cell cultures for transgenesis in other mammalian species has been comparatively nonexistent. By focusing on the laboratory rat as a widely popular model species in science, this chapter highlights several advantages of the spermatogonium as an alternative type of germline stem cell for transgenesis. Protocols for isolating, propagating, genetically modifying, and determining the germline transmission rates of spermatogonial cultures for the production of transgenic rats are introduced in detail. Although the full potential of spermatogonia has yet to be realized in animal genetics, this chapter illustrates how their application as novel germline vectors would open new doors to advance transgenic technology. Most notably, gene manipulations directly in the spermatogonium simplify production of germline founders while bypassing the intermediate production of chimeric progeny using micromanipulated embryos. Once experimental conditions for producing genetically modified animals using spermatogonial cultures are optimized, the approach holds the potential to facilitate targeted germline modifications by gene replacement, gene-insertion and/or restriction endonuclease technologies in a diversity of mammalian species.

Keywords

Seminiferous Tubule Spermatogonial Stem Cell Germline Stem Cell Germline Transmission Matrix Selection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Studies to establish the methodology reported herein was supported by NIH grants R21RR023958 from the National Center for Research Resources and RO1HD036022 from the National Institute of Child Health and Human Development, and by the Cecil H. & Ida Green Center for Reproductive Biology Sciences at the University of Texas Southwestern Medical Center in Dallas.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Karen M. Chapman
    • 1
  • Dalia Saidley-Alsaadi
    • 1
  • Andrew E. Syvyk
    • 1
  • James R. Shirley
    • 1
  • Lindsay M. Thompson
    • 1
  • F. Kent Hamra
    • 1
    Email author
  1. 1.The Department of Pharmacology and the Cecil H. & Ida Green Center for Reproductive Biology SciencesUniversity of Texas Southwestern Medical CenterDallasUSA

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