Abstract
Single-chain Fv fragments exhibiting monovalent binding can be converted into bivalent molecules by reducing the linker connecting the two variable domains to approximately five residues resulting in homodimerization of two polypeptide chains. This protocol describes the cloning steps to convert an scFv into a diabody molecule and provides some information for production and analysis.
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© 2010 Springer-Verlag Berlin Heidelberg
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Kontermann, R.E. (2010). Bivalent Diabodies. In: Kontermann, R., Dübel, S. (eds) Antibody Engineering. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01147-4_5
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DOI: https://doi.org/10.1007/978-3-642-01147-4_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-01146-7
Online ISBN: 978-3-642-01147-4
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