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Antibody Engineering pp 377–386Cite as

Expression of Antibody Fragments in Transgenic Plants

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Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Plants are attractive systems for the expression of full-length antibodies and also for antibody derivatives such as scFvs, diabodies, camelid VHH and minibodies. For ‘Molecular Pharming’ ER retention of antibody fragments has been turned out to be the method of choice for production in leaves as well as in potato tubers and seeds. Various methods have been developed to select and characterize specific and high affine recombinant antibodies against target antigens in plant cells. The necessary technology to accumulate specific antibodies in specific cell compartments or in a plant organ of choice is generally available. Standard methods for expression of recombinant antibody fragments and for their physical and functional characterization are described in this chapter.

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Author information

Authors and Affiliations

  1. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, Gatersleben, Germany

    Udo Conrad

  2. Institute of Biochemistry, Christian Albrechts University, Olshausenstrasse 40, Kiel, Germany

    Doreen M Floss

Authors
  1. Udo Conrad
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  2. Doreen M Floss
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Corresponding author

Correspondence to Udo Conrad .

Editor information

Editors and Affiliations

  1. (Biomedical Engineering) Institut für Zellbiologie und Immunologie, Universität Stuttgart, Allmandring 31, 70569, Stuttgart, Germany

    Roland Kontermann

  2. Technische Universität Braunschweig, Institut für Biochemie und Biotechnologie, Spielmannstraße 7, 38106, Braunschweig, Germany

    Stefan Dübel

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Conrad, U., Floss, D.M. (2010). Expression of Antibody Fragments in Transgenic Plants. In: Kontermann, R., Dübel, S. (eds) Antibody Engineering. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01147-4_29

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  • DOI: https://doi.org/10.1007/978-3-642-01147-4_29

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-01146-7

  • Online ISBN: 978-3-642-01147-4

  • eBook Packages: Springer Protocols

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