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Humanization by Resurfacing

  • Protocol
Antibody Engineering

Part of the book series: Springer Protocols Handbooks ((SPH))

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Monoclonal antibodies can be humanized by changing murine framework surface residues into those that are typically observed in closely related human counterparts. This technique of resurfacing an antibody is based on the observation that the antigenicity of a protein is determined by the accessible and protruding residues. Since nothing is changed to the CDR-framework interactions at the interior, and only subtle changes are made to the exterior, this usually has only minor effects on the conformation and the activity of the resurfaced antibody. A detailed structure-based protocol is proposed. The approach has been successfully applied to two independent antibodies.

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Authors and Affiliations

  1. AlgoNomics NV, Gent, Belgium

    Johan Desmet

  2. Laboratory for Thrombosis Research, KU Leuven campus Kortrijk, E. Sabbelaan 53, 8500, Kortrijk, Belgium

    Karen Vanhoorelbeke & Hans Deckmyn

Authors
  1. Johan Desmet
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  2. Karen Vanhoorelbeke
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  3. Hans Deckmyn
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Corresponding author

Correspondence to Hans Deckmyn .

Editor information

Editors and Affiliations

  1. (Biomedical Engineering) Institut für Zellbiologie und Immunologie, Universität Stuttgart, Allmandring 31, 70569, Stuttgart, Germany

    Roland Kontermann

  2. Technische Universität Braunschweig, Institut für Biochemie und Biotechnologie, Spielmannstraße 7, 38106, Braunschweig, Germany

    Stefan Dübel

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© 2010 Springer-Verlag

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Desmet, J., Vanhoorelbeke, K., Deckmyn, H. (2010). Humanization by Resurfacing. In: Kontermann, R., Dübel, S. (eds) Antibody Engineering. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01144-3_22

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  • DOI: https://doi.org/10.1007/978-3-642-01144-3_22

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-01143-6

  • Online ISBN: 978-3-642-01144-3

  • eBook Packages: Springer Protocols

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