Multicolor FISH using whole-chromosome painting and FISH banding with partial chromosome painting probes are, for technical reasons, not suited to the characterization of small centromeric and pericentromeric rearrangements. Thus, probe sets for characterizing the different human centromeres, and others that enable studies of the near-centromere regions of human chromosomes, were established. Such centromere-specific multicolor (cenM-) and subcentromere-specific (subcenM-) FISH approaches are presented in this chapter. The preparation and use of these probe sets is reported.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Henegariu O, Bray-Ward P, Artan S, Vance GH, Qumsyieh M, Ward DC (2001) Small marker chromosome identification in metaphase and interphase using centromeric multiplex FISH (CM-FISH). Lab Invest 81:475–481
Langer S, Fauth C, Rocchi M, Murken J, Speicher MR (2001) AcroM fluorescent in situ hybridization analyses of marker chromosomes. Hum Genet 109:152–158
Liehr T (2008a) Multicolor FISH homepage. http://www.med.uni-jena.de/fish/mFISH/mFISHlit.htm
Liehr T (2008b) sSMC homepage. http://www.med.uni-jena.de/fish/sSMC/00START.htm
Nietzel A, Rocchi M, Starke H, Heller A, Fiedler W, Wlodarska I, Loncarevic IF, Beensen V, Claussen U, Liehr T (2001) A new multicolor-FISH approach for the characterization of marker chromosomes: Centromere-specific multicolor-FISH (cenM-FISH). Hum Genet 108:199–204
Schröck E, du Manoir S, Veldman T, Schoell B, Wienberg J, Ferguson-Smith MA, Ning Y, Ledbetter DH, Bar-Am I, Soenksen D, Garini Y, Ried T (1996) Multicolor spectral karyotyping of human chromosomes. Science 273:494–497
Speicher MR, Gwyn Ballard S, Ward DC (1996) Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nat Genet 12:368–375
Starke H, Nietzel A, Weise A, Heller A, Mrasek K, Belitz B, Kelbova C, Volleth M, Albrecht B, Mitulla B, Trappe R, Bartels I, Adolph S, Dufke A, Singer S, Stumm M, Wegner RD, Seidel J, Schmidt A, Kuechler A, Schreyer I, Claussen U, von Eggeling F, Liehr T (2003) Small supernumerary marker chromosomes (SMCs): Genotype-phenotype correlation and classification. Hum Genet 114:51–67
Starke H, Mrasek K, Liehr T (2005) 3 cases with enlarged acrocentric p-arms—2 cases with cryptic partial trisomies. J Histochem Cytochem 53:359–360
Trifonov V, Seidel J, Starke H, Martina P, Beensen V, Ziegler M, Hartmann I, Heller A, Nietzel A, Claussen U, Liehr T (2003) Enlarged chromosome 13 p-arm hiding a cryptic partial trisomy 6p22.2-pter. Prenat Diagn 23:427–430
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer-Verlag Berlin Heidelberg
About this protocol
Cite this protocol
Kosyakova, N. et al. (2009). cenM-FISH Approaches. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH) — Application Guide. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70581-9_23
Download citation
DOI: https://doi.org/10.1007/978-3-540-70581-9_23
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-70580-2
Online ISBN: 978-3-540-70581-9
eBook Packages: Springer Protocols