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Antibody Array Generation and Use

  • Carl A. K. Borrebaeck
  • Christer Wingren
Part of the Methods in Molecular Biology book series (MIMB, volume 1131)

Abstract

Affinity proteomics, represented by antibody arrays, is a multiplex technology for high-throughput protein expression profiling of crude proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are individually deposited in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified using mainly fluorescence as the mode of detection. The binding pattern is then converted into a relative protein expression map, or protein atlas, delineating the composition of the sample at the molecular level. The technology provides unique opportunities for various applications, such as protein expression profiling, biomarker discovery, disease diagnostics, prognostics, evidence-based therapy selection, and disease monitoring. Here, we describe the generation and use of planar antibody arrays for serum protein profiling.

Key words

Antibody array Protein expression profiling Microarray Nanoarray Planar arrays Serum profiling 

Notes

Acknowledgements

This work was supported by grants from the Swedish National Science Council (VR-NT and VR-M), the SSF Strategic Center for Translational Cancer Research (CREATE Health), and Vinnova.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Carl A. K. Borrebaeck
    • 1
  • Christer Wingren
    • 1
  1. 1.Department of Immunotechnology and CREATE HealthLund UniversityLundSweden

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