Abstract
The first step in almost every investigation of skeletal phenotypes is analysis of whole-mount skeletal preparations. Whole-mount skeletal staining permits evaluation of the shapes and sizes of skeletal elements in their appropriate locations. The technique is thus the major method for detecting changes in skeletal patterning. Because cartilage and bone can be distinguished by differential staining, this technique is also a powerful means to assess the pace of skeletal maturation. This protocol covers staining of the pre- and postnatal mouse skeleton using Alcian blue and Alizarin red to identify cartilage and bone, respectively.
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References
Horobin RW (2010) How do dyes impart color to different components of the tissues? In: Kumar GL (ed) Educational guide special stains and H & E, 2nd edn. Carpinteria, California, pp 159–166
Schultze O (1897) Ueber herstellung und conservirung durchsichtiger embryonen zum stadium der skeletbildung. Anat Anz 13:3–5
Nagy A, Gerstsenstein M, Vintersten K, Behringer R (2009) Alcian blue staining of the mouse fetal cartilaginous skeleton. Cold Spring Harb Protoc. doi: 10.1101/pdb.prot5169
Jegalian BC, De Robertis EM (1992) Homeotic transformations in the mouse induced by over expression of a human Hox3.3 transgene. Cell 71:901–910
Estrada K, Retting KN, Chin AM et al (2011) Smad6 is essential to limit bmp signaling during cartilage development. J Bone Miner Res 26(10):2498–2510
Nagy A, Gerstsenstein M, Vintersten K, Behringer R (2006) Isolating extraembryonic membranes. Cold Spring Harb Protoc. doi: 10.1101/pdb.prot4267
Hogan BLM, Beddington R, Costantini F, Lacy E (1994) Manipulating the mouse embryo: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Retting KN, Song B, Yoon BS, Lyons KM (2009) BMP and Smad signaling through Smad1 and Smad5 is required for endochondral bone formation. Development 136(7):1093–1104
Ovchinnikov D (2009) Alcian blue/Alizarin red staining of cartilage and bone in mouse. Cold Spring Harb Protoc. doi: 10.1101/pdbprot5170
Nagy A, Gerstsenstein M, Vintersten K, Behringer R (2009) Alizarin red staining of post-natal bone in mouse. Cold Spring Harb Protoc. doi: 10.1101/pdb.prot5171
Green MC (1952) A rapid method for clearing and staining specimens for the demonstration of bone. Ohio J Sci 52(1):31–33
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Rigueur, D., Lyons, K.M. (2014). Whole-Mount Skeletal Staining. In: Hilton, M. (eds) Skeletal Development and Repair. Methods in Molecular Biology, vol 1130. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-989-5_9
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DOI: https://doi.org/10.1007/978-1-62703-989-5_9
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-988-8
Online ISBN: 978-1-62703-989-5
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