Abstract
Spatial and temporal visualization of RNA transcripts in tissue is a key tool in studying both developmental and pathological processes. In situ hybridization is a highly sensitive method for RNA transcript detection. It is based on sequence complementation between a labeled RNA probe and the RNA transcript of interest. The labeled probe is then detected by immunohistochemical methods using an antibody conjugated to an enzyme that catalyzes the generation of chromogenic or fluorescent signals, which indicate the location of the transcript.
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Shwartz, Y., Zelzer, E. (2014). Nonradioactive In Situ Hybridization on Skeletal Tissue Sections. In: Hilton, M. (eds) Skeletal Development and Repair. Methods in Molecular Biology, vol 1130. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-989-5_15
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DOI: https://doi.org/10.1007/978-1-62703-989-5_15
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-988-8
Online ISBN: 978-1-62703-989-5
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