Abstract
Eukaryotic genomes encode a large number of RNA-binding proteins, which play critical roles in many aspects of gene regulation. To functionally characterize these proteins, a key step is to map their interactions with target RNAs. UV crosslinking and immunoprecipitation coupled with high-throughput sequencing has become the standard method for this purpose. Here we describe the detailed procedure that we have used to characterize the protein–RNA interactions of the mRNA 3′ processing factors.
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Acknowledgement
This work was supported by an NIH grant R01GM090056 and ACS grant RSG-12-186.
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Yao, C., Weng, L., Shi, Y. (2014). Global Protein–RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq. In: Hertel, K. (eds) Spliceosomal Pre-mRNA Splicing. Methods in Molecular Biology, vol 1126. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-980-2_27
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DOI: https://doi.org/10.1007/978-1-62703-980-2_27
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-979-6
Online ISBN: 978-1-62703-980-2
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