Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders

  • Houda Zghal Elloumi
  • Steven M. Holland
Part of the Methods in Molecular Biology book series (MIMB, volume 1124)


Inasmuch as neutrophils are the primary cellular defense against bacterial and fungal infections, disorders that affect these white cells typically predispose individuals to severe and recurrent infections. Therefore, diagnosis of such disorders is an important first step in directing long-term treatment/care for the patient. Herein, we describe methods to identify chronic granulomatous disease, leukocyte adhesion deficiency, and neutropenia. The assays are relatively simple to perform and cost effective and can be performed with equipment available in most laboratories.


Chronic granulomatous disease (CGD) Chemiluminescence Leukocyte adhesion deficiency (LAD) Neutropenia Nitroblue tetrazolium Reactive oxygen species Superoxide 


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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Houda Zghal Elloumi
    • 1
  • Steven M. Holland
    • 1
  1. 1.Laboratory of Clinical Infectious DiseasesNational Institute of Allergy and Infectious Diseases, National Institutes of HealthBethesdaUSA

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