High-Purity Neutrophil Isolation from Human Peripheral Blood and Saliva for Transcriptome Analysis
The oral cavity is a source of readily available neutrophils and can be used as a model to better understand the role of neutrophils in chronic inflammatory diseases such as rheumatoid arthritis, bronchitis, periodontitis, and inflammatory bowel disease. In this chapter we describe reproducible methods to obtain highly purified neutrophil samples from blood and saliva in humans to enable cell analysis using whole-genome microarrays.
KeywordsSaliva Oral neutrophils Transcriptome analysis Microarray
This work was funded by The Canadian Institutes of Health Research (CIHR, Ottawa, ON) and the Alpha Omega Foundation of Canada (Toronto, ON). FL is supported by CIHR Training Fellowship, TGF-53877, and the Harron scholarship (Faculty of Dentistry, University of Toronto).