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High-Purity Neutrophil Isolation from Human Peripheral Blood and Saliva for Transcriptome Analysis

  • Flavia S. Lakschevitz
  • Michael Glogauer
Part of the Methods in Molecular Biology book series (MIMB, volume 1124)

Abstract

The oral cavity is a source of readily available neutrophils and can be used as a model to better understand the role of neutrophils in chronic inflammatory diseases such as rheumatoid arthritis, bronchitis, periodontitis, and inflammatory bowel disease. In this chapter we describe reproducible methods to obtain highly purified neutrophil samples from blood and saliva in humans to enable cell analysis using whole-genome microarrays.

Keywords

Saliva Oral neutrophils Transcriptome analysis Microarray 

Notes

Acknowledgments

This work was funded by The Canadian Institutes of Health Research (CIHR, Ottawa, ON) and the Alpha Omega Foundation of Canada (Toronto, ON). FL is supported by CIHR Training Fellowship, TGF-53877, and the Harron scholarship (Faculty of Dentistry, University of Toronto).

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Flavia S. Lakschevitz
    • 1
  • Michael Glogauer
    • 2
  1. 1.Matrix Dynamics Group, Faculty of DentistryUniversity of TorontoTorontoCanada
  2. 2.Department of Periodontology and Matrix Dynamics Group, Faculty of DentistryUniversity of TorontoTorontoCanada

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