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Isolation of Human Neutrophils from Venous Blood

  • William M. Nauseef
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1124)

Abstract

Venous blood provides a ready source of large numbers of unstimulated granulocytes and mononuclear cells. Exploiting the differences in the relative densities of the leukocytes circulating in venous blood, one can separate leukocytes from erythrocytes as well as isolate the individual leukocyte populations in high purity for use in ex vivo studies.

Keywords

Granulocytes Mononuclear cells Ficoll-Hypaque Dextran sedimentation 

References

  1. 1.
    Böyum A (1968) Isolation of mononuclear cells and granulocytes from human blood: isolation of mononuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl 97:77–89PubMedGoogle Scholar
  2. 2.
    Skoog WA, Beck WS (1956) Studies on the fibrinogen, dextran, and phytohemagglutinin methods of isolating leukocytes. Blood 11:436–454PubMedGoogle Scholar
  3. 3.
    Stie J, Jesaitis AJ (2007) Reorganization of the human neutrophil plasma membrane is associated with functional priming: implications for neutrophil preparations. J Leukoc Biol 81:672–685PubMedCrossRefGoogle Scholar
  4. 4.
    Nauseef WN (2007) Isolation of human neutrophils from venous blood. Methods Mol Biol 412:15–20PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • William M. Nauseef
    • 1
  1. 1.Inflammation Program and Department of MedicineRoy J. and Lucille A. Carver College of Medicine, University of IowaCoralvilleUSA

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