Isolation of Human Neutrophils from Venous Blood

  • William M. Nauseef
Part of the Methods in Molecular Biology book series (MIMB, volume 1124)


Venous blood provides a ready source of large numbers of unstimulated granulocytes and mononuclear cells. Exploiting the differences in the relative densities of the leukocytes circulating in venous blood, one can separate leukocytes from erythrocytes as well as isolate the individual leukocyte populations in high purity for use in ex vivo studies.


Granulocytes Mononuclear cells Ficoll-Hypaque Dextran sedimentation 


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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • William M. Nauseef
    • 1
  1. 1.Inflammation Program and Department of MedicineRoy J. and Lucille A. Carver College of Medicine, University of IowaCoralvilleUSA

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