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Ras Signaling pp 207–216Cite as

An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1120))

Abstract

The in vitro kinase assay is an invaluable method to identify and characterize kinase substrates and to determine specific site(s) of phosphorylation. However, the use of recombinant proteins does not adequately account for the contribution of accessory proteins, such as scaffolds, that can contribute to the phosphorylation reaction in vivo. We describe here a method to evaluate the contribution of specific scaffold proteins to kinase reactions using a modified in vitro kinase assay.

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Author information

Authors and Affiliations

  1. Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA

    David F. Kashatus

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  1. David F. Kashatus
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Editor information

Editors and Affiliations

  1. University of Siena, Italy

    Lorenza Trabalzini

  2. University of Torino, Italy

    Saverio Francesco Retta

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Kashatus, D.F. (2014). An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1. In: Trabalzini, L., Retta, S. (eds) Ras Signaling. Methods in Molecular Biology, vol 1120. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-791-4_14

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  • DOI: https://doi.org/10.1007/978-1-62703-791-4_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-790-7

  • Online ISBN: 978-1-62703-791-4

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