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What We Have Learned from Animal Models of HCMV

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1119)

Abstract

Although human cytomegalovirus (HCMV) primary infection is generally asymptomatic, in immune-compromised patients HCMV increases morbidity and mortality. As a member of the betaherpesvirus family, in vivo studies of HCMV are limited due to its species specificity. CMVs from other species are often used as surrogates to express HCMV genes/proteins or used as models for inferring HCMV protein function in humans. Using innovative experiments, these animal models have answered important questions about CMV’s life cycle, dissemination, pathogenesis, immune evasion, and host immune response. This chapter provides CMV biologists with an overview of the insights gained using these animal models. Subsequent chapters will provide details of the specifics of the experimental methods developed for each of the animal models discussed here.

Key words

Human cytomegalovirus Mouse cytomegalovirus Rat cytomegalovirus Rhesus cytomegalovirus Pathogenesis Immune control Immune evasion Dissemination Latency Reactivation Vaccine Animal models for HCMV studies In vivo 
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© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of MicrobiologyUniversity of TennesseeKnoxvilleUSA
  2. 2.Department of MicrobiologyThe University of TennesseeKnoxvilleUSA

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