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Fluorescent Indicators for Functional Optical Imaging

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Optical Imaging of Neocortical Dynamics

Part of the book series: Neuromethods ((NM,volume 85))

Abstract

Optical imaging is developing towards the method of choice for monitoring the activities of large numbers of cells in the cortex of living rodents. During the last decade this approach was fueled by methodological progress in both optical instrumentation and fluorescent indicators of neuronal activities. In the first part of this chapter, we provide information required to reach an informed decision regarding the most suitable fluorescent indicators for the different imaging modalities and the experimental approaches for imaging cortical circuit dynamics in vivo. In particular, an overview is given of the available palettes of synthetic and genetically encoded calcium- and voltage-sensitive indicators. In the second half of the chapter we describe the status of voltage imaging of cortical activity using genetically encoded voltage-sensitive fluorescent proteins.

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Authors and Affiliations

  1. Knöpfel Laboratory for Neuronal Circuit Dynamics, RIKEN Brain Science Institute, Wako, Saitama, Japan

    Walther Akemann & Hiroki Mutoh

  2. RIKEN Brain Science Institute, Wako, Saitama, Japan

    Thomas Knöpfel

  3. Brain Sciences Division, Imperial College, London, UK

    Thomas Knöpfel

Authors
  1. Walther Akemann
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  2. Hiroki Mutoh
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  3. Thomas Knöpfel
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Editors and Affiliations

  1. University of Zurich, Switzerland

    Bruno Weber

  2. University of Zurich, Switzerland

    Fritjof Helmchen

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Akemann, W., Mutoh, H., Knöpfel, T. (2014). Fluorescent Indicators for Functional Optical Imaging. In: Weber, B., Helmchen, F. (eds) Optical Imaging of Neocortical Dynamics. Neuromethods, vol 85. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-785-3_4

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  • DOI: https://doi.org/10.1007/978-1-62703-785-3_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-784-6

  • Online ISBN: 978-1-62703-785-3

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