Optical Imaging of Neocortical Dynamics pp 191-207

Part of the Neuromethods book series (NM, volume 85)

Imaging of Astrocytic Activity in Living Rodents

  • Norio Takata
  • Yoshiaki Shinohara
  • Masamichi Ohkura
  • Tsuneko Mishima
  • Junichi Nakai
  • Hajime Hirase
Protocol

Abstract

Mounting evidence from in vitro experiments supports bidirectional interactions between astrocytes and neurons, suggesting glial involvement in neuronal information processing in the brain. Elevation of the cytosolic calcium ion (Ca2+) concentration has been suggested to be important for gliotransmission; however, the study of Ca2+ dynamics in cerebral cortical astrocytes in vivo became possible only recently. Here, we describe a set of procedures to monitor Ca2+ concentration fluctuations in a population of astrocytes or in astrocytic processes using in vivo two-photon microscopy. Simultaneous recording of neuronal activity by electrodes and astrocytic activity by imaging is a promising way to reveal the nature of neuron–glia interactions in the brain of living rodents.

Key words

Bolus loading Adenovirus G-CaMP Astrocytes Astrocytic processes Cerebral cortex 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Norio Takata
    • 1
  • Yoshiaki Shinohara
    • 1
  • Masamichi Ohkura
    • 2
  • Tsuneko Mishima
    • 1
  • Junichi Nakai
    • 3
  • Hajime Hirase
    • 1
  1. 1.RIKEN Brain Science InstituteWakoJapan
  2. 2.Saitama University, Brain Science Institute SaitamaWakoJapan
  3. 3.Saitama University Brain Science Institute, SaitamaWakoJapan

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