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New Technologies for Ultrahigh-Throughput Genotyping in Plant Taxonomy

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Molecular Plant Taxonomy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1115))

Abstract

Molecular genetic markers represent one of the most powerful tools for the analysis of variation between plant genomes. Molecular marker technology has developed rapidly over the last decade, with the introduction of new DNA sequencing methods and the development of high-throughput genotyping methods. Single nucleotide polymorphisms (SNPs) now dominate applications in modern plant genetic analysis. The reducing cost of DNA sequencing and increasing availability of large sequence data sets permit the mining of this data for large numbers of SNPs. These may then be used in applications such as genetic linkage analysis and trait mapping, diversity analysis, association studies, and marker-assisted selection. Here we describe automated methods for the discovery of SNP molecular markers and new technologies for high-throughput, low-cost molecular marker genotyping. Examples include SNP discovery using autoSNPdb and wheatgenome.info as well as SNP genotyping using Illumina’s GoldenGate™ and Infinium™ methods.

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Author information

Authors and Affiliations

  1. School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, Australia

    David Edwards, Manuel Zander, Jessica Dalton-Morgan & Jacqueline Batley

  2. Australian Centre for Plant Functional Genomics, School of Land Crop and Food Sciences, University of Queensland, Brisbane, QLD, Australia

    David Edwards & Manuel Zander

  3. ARC Centre for Integrated Legume Research, University of Queensland, Brisbane, QLD, Australia

    Manuel Zander, Jessica Dalton-Morgan & Jacqueline Batley

Authors
  1. David Edwards
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  2. Manuel Zander
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  3. Jessica Dalton-Morgan
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  4. Jacqueline Batley
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Editor information

Editors and Affiliations

  1. UMR C53 PVBMT Université de la Réunion-Cirad, Université de la Réunion, Ile de la Réunion, France

    Pascale Besse

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Edwards, D., Zander, M., Dalton-Morgan, J., Batley, J. (2014). New Technologies for Ultrahigh-Throughput Genotyping in Plant Taxonomy. In: Besse, P. (eds) Molecular Plant Taxonomy. Methods in Molecular Biology, vol 1115. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-767-9_8

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  • DOI: https://doi.org/10.1007/978-1-62703-767-9_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-766-2

  • Online ISBN: 978-1-62703-767-9

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