Abstract
Gene targeting is a vital tool for modern biology. The ability to efficiently and repeatedly target the same locus is made more efficient by the site-specific integrase mediated repeated targeting (SIRT) method, which combines homologous recombination, site-specific integration, and bacterial recombineering to conduct targeted modifications of individual loci. Here we describe the recombineering designs and procedures for the introduction of epitope tags, in-frame deletion mutations, and point mutations into plasmids that can later be used for SIRT.
Yi Zhang and William Schreiner contributed equally to this manuscript.
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Acknowledgment
Research in our laboratory is supported by the intramural research program of the National Cancer Institute.
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Zhang, Y., Schreiner, W., Rong, Y.S. (2014). Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophila. In: Storici, F. (eds) Gene Correction. Methods in Molecular Biology, vol 1114. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-761-7_2
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DOI: https://doi.org/10.1007/978-1-62703-761-7_2
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Publisher Name: Humana Press, Totowa, NJ
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