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Isolation of Chromosomal and Plasmid DNA from Staphylococcus epidermidis

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Staphylococcus Epidermidis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1106))

Abstract

The following describes noncommercial methods for the purification of genomic and plasmid DNA from S. epidermidis. These include both large-scale, high molecular weight and quick, small-scale chromosomal DNA extractions, and also a standard alkaline lysis method of plasmid preparation.

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References

  1. Browder HP, Zygmunt WA, Young JR et al (1965) Lysostaphin: enzymatic mode of action. Biochem Biophys Res Commun 19:383–389

    Article  PubMed  CAS  Google Scholar 

  2. Wu JA, Kusuma C, Mond JJ et al (2003) Lysostaphin disrupts Staphylococcus aureus and Staphylococcus epidermidis biofilms on artificial surfaces. Antimicrob Agents Chemother 47:3407–3414

    Article  PubMed  CAS  Google Scholar 

  3. Tipper DJ, Berman MF (1969) Structures of the cell wall peptidoglycans of Staphylococcus epidermidis Texas 26 and Staphylococcus aureus Copenhagen.I. Chain length and average sequence of cross-bridge peptides. Biochemistry 8:2183–2192

    Article  PubMed  CAS  Google Scholar 

  4. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218

    Article  CAS  Google Scholar 

  5. MacNamara PJ (2008) Genetic manipulation in Staphylococcus aureus. In: Lindsay J (ed) Staphylococcus: molecular genetics. Caister Academic Press, Norfolk, UK, pp 89–129

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Pathology and Microbiology, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, NE, USA

    Jill K. Lindgren

Authors
  1. Jill K. Lindgren
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Editor information

Editors and Affiliations

  1. Department of Pathology and Microbiology, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, Nebraska, USA

    Paul D. Fey

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Lindgren, J.K. (2014). Isolation of Chromosomal and Plasmid DNA from Staphylococcus epidermidis . In: Fey, P. (eds) Staphylococcus Epidermidis. Methods in Molecular Biology, vol 1106. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-736-5_9

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  • DOI: https://doi.org/10.1007/978-1-62703-736-5_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-735-8

  • Online ISBN: 978-1-62703-736-5

  • eBook Packages: Springer Protocols

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