Abstract
Staphylococcus epidermidis is now recognized as the primary cause of nosocomial catheter-mediated infections. Bacteria may be introduced exogenously via contamination of the catheter hub or insertion site and endogenously from sepsis. The in vivo model described in this chapter examines the infection resulting from hematogenous seeding of jugular vein catheters.
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References
NNIS (2004) National Nosocomial Infections Surveillance (NNIS) System Report, data summary from January 1992 through June 2004, issued October 2004. Am J Infect Control 32: 470–485
Fey PD (2010) Modality of bacterial growth presents unique targets: how do we treat biofilm-mediated infections? Curr Opin Microbiol 13:610–615
Rupp ME, Ulphani JS, Fey PD et al (1999) Characterization of Staphylococcus epidermidis polysaccharide intercellular adhesin/hemagglutinin in the pathogenesis of intravascular catheter-associated infection in a rat model. Infect Immun 67:2656–2659
Mehall JR, Saltzman DA, Jackson RJ et al (2002) Fibrin sheath enhances central venous catheter infection. Crit Care Med 30:908–912
Ebert T, Smith S, Pancari G et al (2011) Development of a rat central venous catheter model for evaluation of vaccines to prevent Staphylococcus epidermidis and Staphylococcus aureus early biofilms. Hum Vaccin 7:630–638
Chauhan A, Lebeaux D, Decante B et al (2012) A rat model of central venous catheter to study establishment of long-term bacterial biofilm and related acute and chronic infections. PLoS One 7:e37281
Keller JE, Hindman JW, Mehall JR et al (2006) Enoxaparin inhibits fibrin sheath formation and decreases central venous catheter colonization following bacteremic challenge. Crit Care Med 34:1450–1455
Evans GL, Cekoric T Jr, Searcy RL (1968) Comparative effects of anticoagulants on bacterial growth in experimental blood cultures. Am J Med Technol 34:103–112
Sharp PE, La Regina MC (1998) Experimental methodology. In: Suckow MA (ed) The laboratory rat. CRC Press, Boca Raton, pp 129–159
Staszyk C, Bohnet W, Gasse H et al (2003) Blood vessels of the rat tail: a histological re-examination with respect to blood vessel puncture methods. Lab Anim 37:121–125
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Schaeffer, C.R., Woods, K.M., Longo, G.M. (2014). Rat Jugular Catheter Model of Biofilm-Mediated Infection. In: Fey, P. (eds) Staphylococcus Epidermidis. Methods in Molecular Biology, vol 1106. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-736-5_19
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DOI: https://doi.org/10.1007/978-1-62703-736-5_19
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Publisher Name: Humana Press, Totowa, NJ
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