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Molecular Diagnostics for Melanoma pp 601–621Cite as

Quantitative and Spatial Image Analysis of Tumor and Draining Lymph Nodes Using Immunohistochemistry and High-Resolution Multispectral Imaging to Predict Metastasis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1102))

Abstract

Immunohistochemistry is an essential tool for clinical and translational research laboratories. It is mostly used as a qualitative measure of morphology and cell types within tissue. We have developed a high-resolution multispectral imaging method to expand the uses of immunohistochemistry by making it quantitative. In this chapter we describe the technology, both hardware and software, that we use for this method and give examples of applications.

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Acknowledgment

This work was supported by the US National Institute of Health National Cancer Institute grant R01 CA127947-03 and the US Department of Defense Era of Hope grant W81XWH-12-1-0366.

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Authors and Affiliations

  1. Cancer Immunotherapeutics & Tumor Immunology, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, CA, USA

    Kim R. M. Blenman & Peter P. Lee

Authors
  1. Kim R. M. Blenman
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  2. Peter P. Lee
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Editor information

Editors and Affiliations

  1. Cancer Diagnosis Program, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA

    Magdalena Thurin

  2. Sidra Medical and Research Center,Qatar Foundation, Doha, Qatar

    Francesco M. Marincola

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Blenman, K.R.M., Lee, P.P. (2014). Quantitative and Spatial Image Analysis of Tumor and Draining Lymph Nodes Using Immunohistochemistry and High-Resolution Multispectral Imaging to Predict Metastasis. In: Thurin, M., Marincola, F. (eds) Molecular Diagnostics for Melanoma. Methods in Molecular Biology, vol 1102. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-727-3_32

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  • DOI: https://doi.org/10.1007/978-1-62703-727-3_32

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-726-6

  • Online ISBN: 978-1-62703-727-3

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