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Genotyping of Human Leukocyte Antigen (HLA) Ancestral Haplotypes as Prognostic Marker in Cancer Using PCR Analysis

  • Lisa Villabona
  • Emilia Andersson
  • Maddalena Marchesi
  • Giuseppe V. Masucci
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1102)

Abstract

The major histocompatibility complex (MHC) comprises a set of genes that are essential to immunity and surveillance against neoplastic transformation. MHC antigens not only regulate antitumor immune responses in experimental animal models but also directly correlate with survival and prognosis of patients with various types of cancers. Effective recognition of tumor cells by effector T cells may be affected by the genotype and the extent of expression of human leukocyte antigen (HLA)-peptide complexes. Therefore, MHC antigens may serve as potential biomarkers for prognosis and allow selection of cancer patients for specific therapy. We describe PCR-based method to determine the HLA genotype in healthy individuals and patients using blood and tumor tissue as DNA source.

Key words

HLA genotyping Ancestral haplotype 62.1 Malignant melanoma Survival HLA-A typing from formalin-fixed paraffin embedded tissue DNA 

Abbreviations

HLA

Human leucocyte antigen

MHC

Major histocompatibility complex

APM

Antigen presenting machinery

AHH

Ancestral HLA haplotype

PCR

Polymerase chain reaction

MM

Malignant melanoma

EOC

Epithelial ovarian cancer

OS

Overall survival

TTM1

Time from diagnosis to first metastasis

SFM1

Survival time from metastasis

FFPE

Formalin fixed paraffin embedded

PBL

Peripheral blood lymphocytes

Notes

Acknowledgments

These studies were mainly supported by grants from the Cancer Society in Stockholm and the King Gustaf V Jubilee Fund and the Swedish Cancer Society, the Karolinska Institute/Stockholm County ALF grant. Sequences for HLA-A2 primers were kindly provided by Dr. Olle Olerup at Olerup SSP AB.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Lisa Villabona
    • 1
  • Emilia Andersson
    • 1
  • Maddalena Marchesi
    • 1
  • Giuseppe V. Masucci
    • 1
  1. 1.Department of Oncology-PathologyKarolinska Institute, Karolinska University HospitalStockholmSweden

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