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Purification and Functional Analysis of Human Properdin

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1100)

Abstract

Properdin is a member of the alternative pathway of complement. It is unique in that it is the only known positive regulator of the complement system. Properdin can stabilize and promote complement activation, but in addition is also capable of initiating the alternative pathway, making it a unique protein. This chapter focuses on the methods required for purifying human properdin and testing its functionality.

Key words

Properdin Complement Human Anion exchange C3b 
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Notes

Acknowledgments

This work was supported by a grant from the European Union (TranSVIR FP7-PEOPLE-ITN-2008 #238756).

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Department of NephrologyLeiden University Medical CentreLeidenThe Netherlands

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