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Selection of Recombinant Antibodies from Antibody Gene Libraries

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Book cover Gene Function Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1101))

Abstract

Antibodies are indispensable detection reagents for research and diagnostics and represent the biggest class of biological therapeutics on the market. In vitro antibody selection systems offer many advantages over animal-based technologies because the whole selection process is independent of the in vivo immune response. In the last two decades antibody phage display has evolved to the most robust and widely used method and has already yielded thousands of antibodies. The selection of binders by phage display is also referred to as “panning” and based on the specific molecular interaction of antibody phage with an immobilized antigen thus allowing the enrichment and isolation of antigen-specific monoclonal binders from very large antibody gene libraries. Here, we give detailed protocols for the selection of recombinant antibody fragments from antibody gene libraries in microtiter plates.

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Acknowledgements

We gratefully acknowledge the financial support by the FP7 collaborative projects AffinityProteome (contract 222635) and AFFINOMICS (contract 241481). This paper is an updated and revised version of [57, 72, 79].

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  1. Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Braunschweig, Germany

    Michael Hust, André Frenzel, Thomas Schirrmann & Stefan Dübel

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  1. Michael Hust
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  2. André Frenzel
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  1. Dept of Mathematics & Statistics, The College of New Jersey, Ewing, New Jersey, USA

    Michael F. Ochs

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Hust, M., Frenzel, A., Schirrmann, T., Dübel, S. (2014). Selection of Recombinant Antibodies from Antibody Gene Libraries. In: Ochs, M. (eds) Gene Function Analysis. Methods in Molecular Biology, vol 1101. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-721-1_14

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  • DOI: https://doi.org/10.1007/978-1-62703-721-1_14

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