Construction and Application of Site-Specific Artificial Nucleases for Targeted Gene Editing

  • Fatma O. Kok
  • Ankit Gupta
  • Nathan D. Lawson
  • Scot A. Wolfe
Part of the Methods in Molecular Biology book series (MIMB, volume 1101)


Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.

Key words

Zinc finger nucleases ZFNs TALE nucleases TALENs Zebrafish Modular assembly Golden gate assembly 


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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Fatma O. Kok
    • 1
  • Ankit Gupta
    • 1
  • Nathan D. Lawson
    • 1
  • Scot A. Wolfe
    • 1
  1. 1.Program in Gene Function & Expression, Department of Biochemistry & Molecular PharmacologyUMass Medical SchoolWorcesterUSA

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