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Agrobacterium-Mediated Transformation of Maize (Zea mays) Immature Embryos

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1099)

Abstract

Agrobacterium tumefaciens-mediated transformation is one of the most efficient and simple gene delivery systems for genetic improvement and biology studies in maize. This system has become more widely used by both public and private laboratories. However, transformation efficiencies vary greatly from laboratory to laboratory for the same genotype. Here, we illustrate our advanced Agrobacterium-mediated transformation method in Hi-II maize using simple binary vectors. The protocol utilizes immature embryos as starting explants and the bar gene as a selectable marker coupled with bialaphos as a selective agent. The protocol offers efficient transformation results with high reproducibility, provided that some experimental conditions are well controlled. This transformation method, with minor modifications, can be also employed to transform certain maize inbreds.

Keywords

Agrobacterium tumefaciens Hi-II Maize Simple binary vectors bar 
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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Division of Plant SciencesUniversity of MissouriColumbiaUSA

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