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A Protocol for High-Throughput Agrobacterium-Mediated Barley Transformation

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Cereal Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1099))

Abstract

Agrobacterium-mediated transformation of barley is a valuable tool for determining gene function by over-expression of a gene of interest or by RNAi-based gene silencing. The method is based on the inoculation of immature embryos with Agrobacterium and uses a hygromycin resistance gene to allow selection of transgenic plants. The protocol described leads to average transformation efficiencies of 25 % meaning that large numbers of fertile transgenic plants can be produced.

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Harwood, W.A. (2014). A Protocol for High-Throughput Agrobacterium-Mediated Barley Transformation. In: Henry, R., Furtado, A. (eds) Cereal Genomics. Methods in Molecular Biology, vol 1099. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-715-0_20

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  • DOI: https://doi.org/10.1007/978-1-62703-715-0_20

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-714-3

  • Online ISBN: 978-1-62703-715-0

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