Skip to main content

Bacterial Whole-Cell Biosensors for the Detection of Contaminants in Water and Soils

Part of the Methods in Molecular Biology book series (MIMB,volume 1096)

Abstract

Bacterial whole-cell biosensors (BWBs) have unique advantages over conventional environmental monitoring techniques on the detection of toxicity and bioavailability of contaminants in water and soils. BWBs can also be rapid, sensitive, semiquantitative, cost-effective, and easy to use. In this study, a standard method is described for the detection of contaminants and toxicity in real water and soil samples using Acinetobacter baylyi ADP1-based biosensors.

Key words

  • Biosensor
  • Acinetobacter baylyi ADP1
  • Bioluminescence
  • Genotoxicity
  • Environmental samples

This is a preview of subscription content, access via your institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-1-62703-712-9_13
  • Chapter length: 14 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   109.00
Price excludes VAT (USA)
  • ISBN: 978-1-62703-712-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   149.99
Price excludes VAT (USA)
Hardcover Book
USD   219.99
Price excludes VAT (USA)
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Rogers KR (2006) Recent advances in biosensor techniques for environmental monitoring. Anal Chim Acta 568:222–231

    CAS  PubMed  CrossRef  Google Scholar 

  2. Vollmer AC, Van Dyk TK (2004) Stress responsive bacteria: biosensors as environmental monitors. Adv Microb Physiol 49:131–174

    CAS  CrossRef  Google Scholar 

  3. Rodriguez-Mozaz S et al (2005) Biosensors for environmental monitoring—a global perspective. Talanta 65:291–297

    CAS  PubMed  Google Scholar 

  4. Belkin S (2003) Microbial whole-cell sensing systems of environmental pollutants. Curr Opin Microbiol 6:206–212

    CAS  PubMed  CrossRef  Google Scholar 

  5. Rodriguez-Mozaz S, de Alda MJL, Barcelo D (2006) Biosensors as useful tools for environmental analysis and monitoring. Anal Bioanal Chem 386:1025–1041

    CAS  PubMed  CrossRef  Google Scholar 

  6. DeFraia CT, Schmelz EA, Mou ZL (2008) A rapid biosensor-based method for quantification of free and glucose-conjugated salicylic acid. Plant Methods 4:28–38

    PubMed Central  PubMed  CrossRef  Google Scholar 

  7. D’Souza SF (2001) Microbial biosensors. Biosens Bioelectron 16:337–353

    PubMed  CrossRef  Google Scholar 

  8. Mulchandani A, Bassi AS (1995) Principles and applications of biosensors for bioprocess monitoring and control. Crit Rev Biotechnol 15:105–124

    CAS  PubMed  CrossRef  Google Scholar 

  9. Huang WE et al (2006) Quantitative in situ assay of salicylic acid in tobacco leaves using a genetically modified biosensor strain of Acinetobacter sp ADP1. Plant J 46:1073–1083

    CAS  PubMed  CrossRef  Google Scholar 

  10. Huang WE et al (2008) Characterizing the regulation of the Pu promoter in Acinetobacter baylyi ADP1. Environ Microbiol 10: 1668–1680

    CAS  PubMed  CrossRef  Google Scholar 

  11. Zhang D et al (2011) Functionalization of whole-cell bacterial reporters with magnetic nanoparticles. Microb Biotechnol 4:89–97

    CAS  PubMed  CrossRef  Google Scholar 

  12. Zhang D et al (2011) Whole cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills. Microb Biotechnol 5:87–97

    CAS  PubMed  CrossRef  Google Scholar 

  13. Song YZ et al (2009) Optimization of bacterial whole cell bioreporters for toxicity assay of environmental samples. Environ Sci Technol 43:7931–7938

    CAS  PubMed  CrossRef  Google Scholar 

  14. Bauchop T, Elsden SR (1960) The growth of micro-organisms in relation to their energy supply. J Gen Microbiol 23:457–469

    CAS  PubMed  CrossRef  Google Scholar 

  15. Costa P, Manuel J, Lobo S (2001) Modeling and comparison of dissolution profiles. Eur J Pharm Sci 13:123–133

    CAS  PubMed  CrossRef  Google Scholar 

  16. Meighen EA (1991) Molecular-biology of bacterial bioluminescence. Microbiol Revs 55:123–142

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2014 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Wang, Y., Zhang, D., Davison, P.A., Huang, W.E. (2014). Bacterial Whole-Cell Biosensors for the Detection of Contaminants in Water and Soils. In: Paulsen, I., Holmes, A. (eds) Environmental Microbiology. Methods in Molecular Biology, vol 1096. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-712-9_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-712-9_13

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-711-2

  • Online ISBN: 978-1-62703-712-9

  • eBook Packages: Springer Protocols