Abstract
The generation of high-resolution maps of the epigenome is crucial to research in epigenetics, developmental biology, and stem cell biology. In recent years, small RNA pathways have been implicated in epigenetic regulation. All small RNA pathways involve Argonaute proteins as their key biogenesis and effector components. In this chapter, we describe a chromatin immunoprecipitation method for whole-genome mapping of Drosophila Piwi, the defining member of the Argonaute protein family. This method should have general utility for mapping other chromatin-associated factors.
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Acknowledgment
The development of the method described here was supported by NIH DP1CA174418 and an Ellison Medical Foundation Senior Scholar Award. We thank Ms. Ah Rume (julie) Park for valuable suggestions and Dr. Nils Neuenkirchen for proof-reading the manuscript.
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Yin, H., Lin, H. (2014). Chromatin Immunoprecipitation Assay of Piwi in Drosophila . In: Siomi, M. (eds) PIWI-Interacting RNAs. Methods in Molecular Biology, vol 1093. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-694-8_1
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DOI: https://doi.org/10.1007/978-1-62703-694-8_1
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-693-1
Online ISBN: 978-1-62703-694-8
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