Abstract
In order to highlight our understanding on ecosystems functioning and resource sharing/competition, either in artificial environment or agrosystems, according to changes in the climatic conditions, it is necessary to measure accurately element fluxes within plants. Stable isotopes allow tracking safely and accurately on a short time frame the behavior of elements in plants. After a short review devoted to isotopic studies of elemental flux within plants, we explain how a direct multiple labelling study might be conducted in a plant, so as to measure over short time nitrogen and sulfur acquisition, and assimilates arising from a labelled source.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Good AG, Johnson SJ, De Pauw M et al (2007) Engineering nitrogen use efficiency with alanine aminotransferase. Can J Bot 85:252–262
Masclaux-Daubresse C, Daniel-Vedele F, Dechorgnat J et al (2010) Nitrogen uptake, assimilation and remobilization in plants: challenges for sustainable and productive agriculture. Ann Bot 105:1141–1157
Kant S, Bi YM, Rothstein SJ (2011) Understanding plant response to nitrogen limitation for the improvement of crop nitrogen use efficiency. J Exp Bot 62:1499–1509
Salon C, Avice JC, Larmure A et al (2011) Plant N fluxes and modulation by nitrogen, heat and water stresses: a review based on comparison of legumes and non legume plants. In: INTECH (eds) Abiotic stress in plants: mechanisms and adaptations, http://www.intechweb.org/
Schiltz S, Munier-Jolain NG, Jeudy C et al (2005) Dynamic of exogenous nitrogen partitioning and nitrogen remobilisation from vegetative organs in pea revealed by 15N in vivo labeling throughout seed filling. Plant Physiol 137:1463–1473
Desclos M, Etienne P, Coquet L et al (2009) A combined 15N tracing/proteomics study in Brassica napus reveals the chronology of proteomics events associated with N remobilization during leaf senescence induced by nitrate limitation or starvation. Proteomics 9:3580–3608
Noquet C, Avice JC, Rossato L et al (2004) Effects of altered source-sink relationships on N allocation and vegetative storage protein accumulation in Brassica napus L. Plant Sci 166:1007–1018
Larmure A, Salon C, Munier-Jolain NG (2005) How does temperature affect C and N allocation to the seeds during the seed filling period in pea? Effect on seed nitrogen concentration. Funct Plant Biol 32:1009–1017
Schnug E, Haneklaus S, Murphy D (1993) Impact of sulphur fertilization on fertilizer nitrogen efficiency. Sulphur Agric 17:8–12
Fismes J, Vong PC, Guckert A, Frossard E (2000) Influence of sulfur on apparent N-use efficiency, yield and quality of oilseed rape (Brassica napus L.) grown on a calcareous soil. Eur J Agron 12:127–141
Dubousset L, Etienne P, Avice J-C (2010) Is the remobilisationof S- and N-reserves for seed filling of winter oilseed rape modulated by sulphate restrictions occurring at different growth stages? J Exp Bot 61:4313–4324
Kopriva S, Rennenberg H (2004) Control of sulphate assimilation and glutathione synthesis: interaction with N and C metabolism. J Exp Bot 55:1831–1842
Scherer HW, Pacyna S, Manthey N, Schulz M (2006) Sulphur supply to peas (Pisum sativum L.) influences symbiotic N2 fixation. Plant Soil Environ 52:72–77
Varin S, Cliquet J-B, Personeni E, Avice J-C, Lemauviel-Lavenant S (2010) How does sulphur availability modify N acquisition of White Clover (Trifolium repens L.)? J Exp Bot 61:225–234
Salon C, Raymond P, Pradet A (1988) Quantification of carbon fluxes through the tricarboxylic acid cycle in early germinating lettuce embryos. J Biol Chem 263:12278–12287
Avice J-C, Ourry A, Lemaire G, Boucaud J (1996) Nitrogen and carbon flows estimated by 15N and 13C pulse chase labelling during regrowth of alfalfa. Plant Physiol 112(1):281–290
Voisin AS, Salon C, Munier-Jolain NG et al (2002) Quantitative effect of soil nitrate, growth potential and phenology on symbiotic nitrogen fixation of pea (Pisum sativum L.). Plant and Soil 243:31–42
Moreau D, Bourion V, Fournier H et al (2006) Medicaco truncatula protocols: phenotyping. In: Malthesius U, Journet EP, Sumner LW (eds) The medicago truncatula hand book, http://www.noble.org/MedicagoHandbook/. ISBN: 0-9754303-1-9
Voisin AS, Salon C, Amarger N et al (2008) Theoretical evaluation of isotopic 15N methods for measurement of symbiotic nitrogen fixation in the field. In: Couto GN (ed) Nitrogen fixation research progress. Nova Science Publishers Inc, New York, NY, pp 331–354
Voisin AS, Salon C, Jeudy C et al (2003) Symbiotic N2 fixation in relation to C economy of Pisum sativum L. as a function of plant phenology. J Exp Bot 54:2733–2744
Malagoli P, Lainé P, Rossato L, Ourry A (2005) Dynamic of nitrogen uptake and mobilisation in field grown winter oilseed rape from stem extension to harvest. I. Global N flows between vegetative and reproductive tissues in relation with leaf fall and their residual N. Ann Bot 95:853–861
Munier-Jolain NG, Salon C (2003) Can sucrose content in phloem sap reaching field pea (Pisum sativum L.) seeds be a useful indicator of seed growth potential? J Exp Bot 54(392):2457–2465
Lepinay C, Rigaud T, Salon C et al (2012) Interaction between Medicago truncatula and Pseudomonas fluorescens C7R12: evaluation of costs and benefits across an elevated atmospheric CO2. PLoS ONE 7(9):e45740
Zancarini A, Mougel C, Voisin AS et al (2012) Soil Nitrogen Availability Modifies the Nutrition Strategies of the Interaction between Medicago truncatula and the Rhizosphere Bacterial Communities. PLoS ONE 7(10):e47096
Jeudy C, Ruffel S, Freixes S et al (2010) Plasticity of nodule development has a major role in the adaptation of Medicago truncatula to N-limitation. New Phytol 185:817–828
Ruffel S, Freixes S, Balzergue S et al (2008) Systemic signalling of the plant N status triggers specific transcriptome responses depending on the N source in Medicago Truncatula. Plant Physiol 146:2020–2035
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Salon, C. et al. (2014). 34S and 15N Labelling to Model S and N Flux in Plants and Determine the Different Components of N and S Use Efficiency. In: Dieuaide-Noubhani, M., Alonso, A. (eds) Plant Metabolic Flux Analysis. Methods in Molecular Biology, vol 1090. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-688-7_20
Download citation
DOI: https://doi.org/10.1007/978-1-62703-688-7_20
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-687-0
Online ISBN: 978-1-62703-688-7
eBook Packages: Springer Protocols