Abstract
Vectors derived from the canine adenovirus serotype 2 (CAV-2) possess a high neurotropism and efficient retrograde transport that lead to widespread neuronal transduction in the central nervous system (CNS) of various animals. These abilities are due to the engagement of virions to the coxsackievirus and adenovirus receptor at the surface of neurons, which is linked to the endocytic and axonal transport machineries. The trafficking of CAV-2 and the coxsackievirus and adenovirus receptor (CAR) can be visualized ex vivo by incubating primary neurons (e.g., motoneurons and hippocampal neurons) with fluorescently labeled virions or recombinant viral proteins. Using this approach, we could recapitulate the mechanisms responsible for long-range transport of adenovirus in neurons.
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Acknowledgments
We thank Eric Kremer and members of the EKL group, G. Schiavo, and G. Menendez for the microfluidic chambers. S.S. is an Institut National de la Santé et de la Recherche Médicale (INSERM fellow). Work in the laboratory is funded by the European Community’s 7th Framework Programme (FP7/2007–2013; grant 222992—BrainCAV), the French Agence National de la Recherche, E-Rare, the Fondation de France and the Association Française contre les Myopathies.
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Zussy, C., Salinas, S. (2014). Study of Adenovirus and CAR Axonal Transport in Primary Neurons. In: Chillón, M., Bosch, A. (eds) Adenovirus. Methods in Molecular Biology, vol 1089. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-679-5_5
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DOI: https://doi.org/10.1007/978-1-62703-679-5_5
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