Abstract
The study of biologically relevant native RNA structures is important to understand their cellular function(s). Native gel electrophoresis provides information about such native structures in solution as a function of experimental conditions. The application of native gel electrophoresis in a comparative manner allows to obtain precise information on relative angles subtended between given pair of stems in an RNA molecule. By adapting this approach, it is possible to obtain very specific structural information such as the amplitude of dihedral angles and helical rotation. As an example, we will describe how native gel electrophoresis can be used to study the folding of the S-adenosylmethionine (SAM) sensing riboswitch.
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Acknowledgements
This work was supported by the National Sciences and Engineering Research Council of Canada (NSERC). DAL is a Canadian Institutes of Health Research (CIHR) New Investigator Scholar.
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Eschbach, S.H., Lafontaine, D.A. (2014). RNA Conformational Changes Analyzed by Comparative Gel Electrophoresis. In: Waldsich, C. (eds) RNA Folding. Methods in Molecular Biology, vol 1086. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-667-2_15
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DOI: https://doi.org/10.1007/978-1-62703-667-2_15
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