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Subpicosecond Kerr-Gate Spectrofluorometry

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Fluorescence Spectroscopy and Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1076))

Abstract

This chapter describes an experimental layout for time and spectrally resolved fluorescence measurements with femtosecond time resolution based on Kerr gating. The combination of data recorded using different Kerr media allows a temporal dynamic range from ~100 fs to several nanoseconds. Simultaneous analysis of multiple datasets is described.

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Author information

Authors and Affiliations

  1. Laboratoire d’Optique et Biosciences, INSERM U696-CNRS UMR7645, Ecole Polytechnique, Palaiseau, France

    Sergey P. Laptenok, Patrick Nuernberger, Andras Lukacs & Marten H. Vos

  2. Institut für Physikalische und Theoretische Chemie, Julius-Maximilians-Universität Würzburg, Würzburg, Germany

    Patrick Nuernberger

  3. Department of Biophysics, Medical School, University of Pécs, Pécs, Hungary

    Andras Lukacs

Authors
  1. Sergey P. Laptenok
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  2. Patrick Nuernberger
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  3. Andras Lukacs
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  4. Marten H. Vos
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Editor information

Editors and Affiliations

  1. KULeuven-Biomolecular Dynamics BioSCENTer, Leuven, Belgium

    Yves Engelborghs

  2. MicroSpectroscopy Centre, University of Wageningen Biochemistry, Wageningen, The Netherlands

    Antonie J.W.G. Visser

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Laptenok, S.P., Nuernberger, P., Lukacs, A., Vos, M.H. (2014). Subpicosecond Kerr-Gate Spectrofluorometry. In: Engelborghs, Y., Visser, A. (eds) Fluorescence Spectroscopy and Microscopy. Methods in Molecular Biology, vol 1076. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-649-8_13

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  • DOI: https://doi.org/10.1007/978-1-62703-649-8_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-648-1

  • Online ISBN: 978-1-62703-649-8

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