Abstract
The cortical microtubules, and to some extent also the actin meshwork, play a central role in the shaping of plant cells. Transgenic plants expressing fluorescent protein markers specifically tagging the two main cytoskeletal systems are available, allowing noninvasive in vivo studies. Advanced microscopy techniques, in particular confocal laser scanning microscopy (CLSM) and variable angle epifluorescence microscopy (VAEM), can be nowadays used for imaging the cortical cytoskeleton of living cells with unprecedented spatial and temporal resolution. With the aid of suitable computing techniques, quantitative information can be extracted from microscopic images and video sequences, providing insight into both architecture and dynamics of the cortical cytoskeleton.
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Acknowledgments
This work has been supported by the GAČR P305/10/0433 project. We thank Boris Voigt and Richard Cyr for transgenic Arabidopsis lines; Ondřej Šebesta, Ondřej Horváth, and Aleš Soukup for expert microscopy advice; and Marta Čadyová for technical assistance.
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Rosero, A., Žárský, V., Cvrčková, F. (2014). Visualizing and Quantifying the In Vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton Using CLSM and VAEM. In: Žárský, V., Cvrčková, F. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1080. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-643-6_7
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DOI: https://doi.org/10.1007/978-1-62703-643-6_7
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