Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons

  • Armine Darbinyan
  • Rafal Kaminski
  • Martyn K. White
  • Nune Darbinian
  • Kamel Khalili
Part of the Methods in Molecular Biology book series (MIMB, volume 1078)


The research on human neural progenitor cells holds great potential for the understanding the molecular programs that control differentiation of cells of glial and neuronal lineages and pathogenetic mechanisms of neurological diseases. Stem cell technologies provide also opportunities for pharmaceutical industry to develop new approaches for regenerative medicine. Here we describe the protocol for isolation and maintenance of neural progenitor cells and cortical neurons using human fetal brain tissue. This protocol can be successfully adapted for preparation of rodent neural and oligodendrocyte progenitor cells. While several methods for isolation of neural and oligodendrocyte progenitors from rodent brain tissue have been described, including techniques which use gene transfer and magnetic resonance beads, few methods are focused on derivation of human oligodendrocyte progenitor cells. Development of human culture provides the most physiologically relevant system for investigation of mechanisms which regulate function of oligodendrocyte, specifically of human origin.

Key words

Human neural progenitor cells Neurosphere Oligodendrocyte progenitor cells Neuron Oligodendrocyte 


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Armine Darbinyan
    • 1
  • Rafal Kaminski
    • 2
  • Martyn K. White
    • 2
  • Nune Darbinian
    • 2
  • Kamel Khalili
    • 2
  1. 1.Department of PathologyThe Mount Sinai Medical CenterNew YorkUSA
  2. 2.Department of NeuroscienceTemple University School of MedicinePhiladelphiaUSA

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