Abstract
This chapter describes the culture and propagation of murine embryonic stem cells, F9 and P19 and strategies for differentiation of these stem cells into neurons. Protocols focus on maintenance and propagation of these cells and routine procedures employed for differentiation into neuronal cells. Additional protocols are also described for obtaining enriched populations of mature neurons from P19 cells and differentiation of F9 cells into serotonergic or catecholaminergic neurons.
The protocols described herein can be employed for dissection of the pathways such as gliogenesis and neurogenesis that are involved in differentiation of pluripotent stem cells such as F9 and P19 into glial cells or terminally differentiated neurons.
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Acknowledgments
I am grateful to Dr. Ron Reichel for introducing me to the field of embryonic stem cells and differentiation during the early years of my career. Studies in the author’s laboratory are funded by a grant from NIH/NIDA.
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Datta, P.K. (2013). Murine Teratocarcinoma-Derived Neuronal Cultures. In: Amini, S., White, M. (eds) Neuronal Cell Culture. Methods in Molecular Biology, vol 1078. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-640-5_4
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DOI: https://doi.org/10.1007/978-1-62703-640-5_4
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