Abstract
The use of sensory neurons and assessment of neurite outgrowth in vitro is an important part of understanding neuronal development and plasticity. Cultures of rat dorsal root ganglion (DRG) neurons provide quantitative results very quickly and, when grown on growth promoting or inhibitory substrates, can be utilized to study axonal growth, neurotrophic dependence, structure and function of growth cones. Since we are interested in axon regeneration and targeting, we have sought to promote neurite outgrowth by refining the techniques of growing DRG neurons in culture. This chapter describes detailed methods for the dissection and purification of DRG neurons and quantitative assessment of neurite on promoting or inhibitory substrates.
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Acknowledgment
This work was supported by NIH grant RO1 NS060784, Shriners Hospitals for Children (GMS) and Shriners Postdoctoral Fellowship (G.M.S. and Y.L.).
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Smith, G.M., Liu, Y., Hong, J.W. (2013). Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates. In: Amini, S., White, M. (eds) Neuronal Cell Culture. Methods in Molecular Biology, vol 1078. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-640-5_14
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DOI: https://doi.org/10.1007/978-1-62703-640-5_14
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-639-9
Online ISBN: 978-1-62703-640-5
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