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Transfection of Neuronal Cultures

  • Ilker Kudret Sariyer
Part of the Methods in Molecular Biology book series (MIMB, volume 1078)

Abstract

Efficient transfection of genes into neurons is a crucial step for the study of neuronal cell biology and functions. These include but are not limited to investigating gene function by overexpression of target proteins via expression plasmids and knocking down the expression levels of neuronal genes by RNA interference (RNAi). In addition, reporter gene constructs are widely used to investigate the promoter activities of neuronal genes. Numerous transfection techniques have been established to deliver genes into the cells. However, efficient transfection of post-mitotic cells, including neurons, still remains a challenging task. Here, we overview the advantages and disadvantages of various techniques for the transfection of primary neurons, and provide an optimized protocol for FuGENE-6 (Promega) which allows a suitable transfection efficiency of primary neuronal cultures.

Key words

Primary neurons Gene delivery Transfection Electroporation Nucleofection Calcium co-precipitation Lipofection FuGENE-6 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Ilker Kudret Sariyer
    • 1
  1. 1.Department of NeuroscienceTemple University School of MedicinePhiladelphiaUSA

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