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Sirtuins pp 39–56Cite as

C. elegans Sirtuins

Part of the Methods in Molecular Biology book series (MIMB,volume 1077)

Abstract

The nematode Caenorhabditis elegans (C. elegans) has four Sir2 paralogs, sir-2.1, sir-2.2, sir-2.3, and sir-2.4. Thus far, most of the research tools to study worm sirtuins have been developed for sir-2.1, due to its homology to yeast SIR2 and human SIRT1. Here, we have compiled a listing of the currently available strains (including both loss-of-function alleles and transgenics), antibodies, and RNAi constructs relevant to studies on all C. elegans sirtuin family members. We also describe the methods used in the analysis of C. elegans sirtuin function, including life span analysis, various stress-resistance assays, and fat content analysis and provide an overview of all phenotypic data relevant to C. elegans sir-2.1.

Key words

  • C. elegans
  • sirtuin
  • sir-2.1
  • sir-2.2
  • sir-2.3
  • sir-2.4
  • Resveratrol

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Author information

Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA

    Mohan Viswanathan

  2. Program in Gene Function and Expression, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA

    Heidi A. Tissenbaum

Authors
  1. Mohan Viswanathan
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  2. Heidi A. Tissenbaum
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Editor information

Editors and Affiliations

  1. Duke University Medical Center Institute of Molecular Physiology, Durham, North Carolina, USA

    Matthew D. Hirschey

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Viswanathan, M., Tissenbaum, H.A. (2013). C. elegans Sirtuins. In: Hirschey, M. (eds) Sirtuins. Methods in Molecular Biology, vol 1077. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-637-5_3

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  • DOI: https://doi.org/10.1007/978-1-62703-637-5_3

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