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Sirtuins in Yeast: Phenotypes and Tools

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Sirtuins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1077))

Abstract

Originally discovered as a transcriptional silencing protein, SIR2 was later linked to yeast replicative aging and the rest was history. Sir2p is now known to be a member of a class of protein deacetylases with a unique enzymatic activity coupling the deacetylation event to NAD+ hydrolysis. While still incompletely understood, the mechanism by which Sir2p modulates yeast aging is linked to inhibition of rDNA recombination. Here we describe phenotypes associated with yeast Sirtuins and assays used to monitor Sirtuin function in yeast, including the replicative aging assay.

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Author information

Authors and Affiliations

  1. Buck Institute for Research on Aging, Novato, CA, USA

    Scott Tsuchiyama & Brian K. Kennedy

  2. Department of Genome Sciences, University of Washington, Seattle, WA, USA

    Elizabeth Kwan

  3. Fred Hutchinson Cancer Research Center, Seattle, WA, USA

    Elizabeth Kwan & Antonio Bedalov

  4. Huffington Center on Aging, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA

    Weiwei Dang

Authors
  1. Scott Tsuchiyama
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  2. Elizabeth Kwan
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  3. Weiwei Dang
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  4. Antonio Bedalov
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  5. Brian K. Kennedy
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Editor information

Editors and Affiliations

  1. Duke University Medical Center Institute of Molecular Physiology, Durham, North Carolina, USA

    Matthew D. Hirschey

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© 2013 Springer Science+Business Media, LLC

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Tsuchiyama, S., Kwan, E., Dang, W., Bedalov, A., Kennedy, B.K. (2013). Sirtuins in Yeast: Phenotypes and Tools. In: Hirschey, M. (eds) Sirtuins. Methods in Molecular Biology, vol 1077. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-637-5_2

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  • DOI: https://doi.org/10.1007/978-1-62703-637-5_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-636-8

  • Online ISBN: 978-1-62703-637-5

  • eBook Packages: Springer Protocols

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