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Making a Protein Extract from Plant Pathogenic Fungi for Gel- and LC-Based Proteomics

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Book cover Plant Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1072))

Abstract

Proteomic technologies have become a successful tool to provide relevant information on fungal biology. In the case of plant pathogenic fungi, this approach would allow a deeper knowledge of the interaction and the biological cycle of the pathogen, as well as the identification of pathogenicity and virulence factors. These two elements open up new possibilities for crop disease diagnosis and environment-friendly crop protection. Phytopathogenic fungi, due to its particular cellular characteristics, can be considered as a recalcitrant biological material, which makes it difficult to obtain quality protein samples for proteomic analysis. This chapter focuses on protein extraction for gel- and LC-based proteomics with specific protocols of our current research with Botrytis cinerea.

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Acknowledgements

This work was supported by the Spanish Ministry of Science and Innovation (BotBank Project, EUI2008-03686), the Andalusian Regional Government (Junta de Andalucía), and the University of Córdoba (AGR-0164: Agricultural and Plant Biochemistry and Proteomics Research Group).

Author information

Authors and Affiliations

  1. Agroforestry and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence, Córdoba, Spain

    Raquel González Fernández

  2. Agroforestry and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence, ceiA3, Córdoba, Spain

    Inmaculada Redondo & Jesus V. Jorrin-Novo

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  1. Raquel González Fernández
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  2. Inmaculada Redondo
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  3. Jesus V. Jorrin-Novo
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Editor information

Editors and Affiliations

  1. Dept of Biochem & Molecular Bio, University of Cordoba, Cordoba, Spain

    Jesus V. Jorrin-Novo

  2. University of Tsukuba National Institute of Crop Science, Tsukuba, Japan

    Setsuko Komatsu

  3. Molecular Systems Biology, University of Vienna, Vienna, Austria

    Wolfram Weckwerth

  4. Dept. Molecular Systems Biology, University of Vienna, Vienna, Austria

    Stefanie Wienkoop

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Fernández, R.G., Redondo, I., Jorrin-Novo, J.V. (2014). Making a Protein Extract from Plant Pathogenic Fungi for Gel- and LC-Based Proteomics. In: Jorrin-Novo, J., Komatsu, S., Weckwerth, W., Wienkoop, S. (eds) Plant Proteomics. Methods in Molecular Biology, vol 1072. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-631-3_8

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  • DOI: https://doi.org/10.1007/978-1-62703-631-3_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-630-6

  • Online ISBN: 978-1-62703-631-3

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