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Epiblast Stem Cells pp 1–13Cite as

Generation and Characterization of Epiblast Stem Cells from Blastocyst-Stage Mouse Embryos

Part of the Methods in Molecular Biology book series (MIMB,volume 1074)

Abstract

Mouse epiblast stem cells (EpiSCs) are pluripotent embryonic cells that can be used to interrogate developmental transitions that occur during gastrulation. EpiSCs can also be robustly differentiated into functional somatic and germ cell derivatives making them a useful tool for studying development and regenerative medicine. Typically, mouse EpiSCs are isolated from the early postimplantation epiblast around 5.5 days post coitum (dpc). This chapter describes the methods for isolation of mouse EpiSCs from preimplantation blastocyst-stage mouse embryos (3.5 dpc). This technique enables the routine ability to derive EpiSC lines as it is much less labor intensive than isolation of EpiSCs from the postimplantation epiblast. We also detail relevant assays used to characterize new EpiSC lines and distinguish them from mouse embryonic stem cells.

Key words

  • Epiblast
  • Pluripotency
  • Stem cell
  • Immunostaining
  • RNA-seq
  • Differentiation

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  • DOI: 10.1007/978-1-62703-628-3_1
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Author information

Authors and Affiliations

  1. Department of Genetics and Genome Sciences, Case Western Reserve, University School of Medicine, Cleveland, OH, USA

    Daniel C. Factor, Fadi J. Najm & Paul J. Tesar

  2. Department of Neurosciences, Case Western Reserve University School of Medicine, Cleveland, OH, USA

    Paul J. Tesar

Authors
  1. Daniel C. Factor
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  2. Fadi J. Najm
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  3. Paul J. Tesar
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Editor information

Editors and Affiliations

  1. School of Biosciences Rm 223 South Lab, University of Nottingham, Loughborough, United Kingdom

    Ramiro Alberio

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Factor, D.C., Najm, F.J., Tesar, P.J. (2013). Generation and Characterization of Epiblast Stem Cells from Blastocyst-Stage Mouse Embryos. In: Alberio, R. (eds) Epiblast Stem Cells. Methods in Molecular Biology, vol 1074. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-628-3_1

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  • DOI: https://doi.org/10.1007/978-1-62703-628-3_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-627-6

  • Online ISBN: 978-1-62703-628-3

  • eBook Packages: Springer Protocols