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Stepwise Differentiation from Naïve State Pluripotent Stem Cells to Functional Primordial Germ Cells Through an Epiblast-Like State

  • Katsuhiko Hayashi
  • Mitinori Saitou
Part of the Methods in Molecular Biology book series (MIMB, volume 1074)

Abstract

A group of pluripotent cells appearing during mammalian embryogenesis is the source for all the cell lineages that compose the embryo proper. In mice, pluripotent cells are first established in the inner cell mass (ICM) of the preimplantation blastocyst. After implantation, the ICM soon transforms into a cup-shaped epithelium, called the postimplantation epiblast. The two types of pluripotent cells, the ICM and postimplantation epiblast cells, are distinct, based on the differences in their gene expression profiles, epigenetic status, and differentiation capacity. During gastrulation, some of the postimplantation epiblast cells adjacent to the extraembryonic ectoderm are specified as primordial germ cells (PGCs), precursors of the germ cell lineage, in response to bone morphogenetic protein 4 (BMP4). Recently, we succeeded in reconstituting epiblast differentiation and PGC specification in vitro using pluripotent stem cells. Here, we describe the culture method of a stepwise differentiation from pluripotent stem cells to functional PGCs.

Key words

Embryonic stem cells Inner cell mass Postimplantation epiblast Primordial germ cells BMP4 

Notes

Acknowledgments

We would like to thank Drs. Hiroshi Ohta and Kazuki Kurimoto for their contribution to establishing this culture system. This study was supported, in part, by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan; by PRESTO; by JST-CREST/ERATO; and by the Takeda Science Foundation. We apologize to researchers whose work has not been cited due to space limitations.

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Katsuhiko Hayashi
    • 1
  • Mitinori Saitou
    • 2
  1. 1.Department of Anatomy and Cell Biology, Graduate School of MedicineKyoto UniversityKyotoJapan
  2. 2.Department of Anatomy and Cell biology, Graduate School of MedicineKyoto UniversityKyotoJapan

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