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Synthetic Biology pp 75–84Cite as

In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1073))

Abstract

Bacterial noncoding small RNAs (sRNAs) modulate expression of numerous genes through antisense interactions with mRNAs. This chapter describes an in vivo screening strategy to engineer artificial sRNAs that can posttranscriptionally regulate desired endogenous genes in Escherichia coli. Artificial sRNA libraries are constructed by randomizing the antisense domain of natural sRNAs and screened for gene silencing activity using a cotransformed reporter vector. These small synthetic riboregulators can be used in synthetic gene circuits to control cell functions by directly targeting endogenous genes.

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Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, University of California, Davis, CA, USA

    Vandana Sharma & Yohei Yokobayashi

Authors
  1. Vandana Sharma
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  2. Yohei Yokobayashi
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Editor information

Editors and Affiliations

  1. Division of Molecular Biosciences, Imperial College London, London, United Kingdom

    Karen M. Polizzi

  2. Department of Chemical Engineering, Imperial College London, London, United Kingdom

    Cleo Kontoravdi

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© 2013 Springer Science+Business Media, New York

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Sharma, V., Yokobayashi, Y. (2013). In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli . In: Polizzi, K., Kontoravdi, C. (eds) Synthetic Biology. Methods in Molecular Biology, vol 1073. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-625-2_8

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  • DOI: https://doi.org/10.1007/978-1-62703-625-2_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-624-5

  • Online ISBN: 978-1-62703-625-2

  • eBook Packages: Springer Protocols

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