Creation and Characterization of Component Libraries for Synthetic Biology

  • Tim Weenink
  • Tom Ellis
Part of the Methods in Molecular Biology book series (MIMB, volume 1073)

Abstract

Large numbers of well-described components are essential for advanced synthetic biology and model-guided design of pathways and regulatory networks. Here a method is presented for the creation of libraries of novel control elements. From these libraries, parts with well-defined properties can be selected and used in construction of finely tuned synthetic systems. The example of the PFY1 promoter in S. cerevisiae is used to describe library creation using degenerate synthetic oligos and the circular polymerase extension cloning (CPEC) method. Additionally the workflow of screening the raw library for functional parts is included to provide a full overview of the process of creating and characterizing a component library for synthetic biology.

Key words

Components Parts Libraries Screening Synthetic biology Characterization Regulation Promoter Yeast Engineering 

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Tim Weenink
    • 1
    • 2
  • Tom Ellis
    • 1
    • 2
  1. 1.Centre for Synthetic Biology and InnovationImperial College LondonLondonUK
  2. 2.Department of BioengineeringImperial College LondonLondonUK

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