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A Multiparameter Live Cell Imaging Approach to Monitor Cyclic AMP and Protein Kinase A Dynamics in Parallel

  • Nwe-Nwe Aye-Han
  • Jin Zhang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1071)

Abstract

Parallel detection of signaling activities allows us to correlate activity dynamics between signaling molecules. In this review, we detail a multiparameter live cell imaging method to monitor 3′,5′-cyclic adenosine monophosphate (cAMP) levels and protein kinase A (PKA) activities in parallel.

Key words

Parallel imaging FRET cAMP Protein kinase A Signal transduction 

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Nwe-Nwe Aye-Han
    • 1
  • Jin Zhang
    • 1
  1. 1.Department of Pharmacology and Molecular SciencesThe Johns Hopkins University School of MedicineBaltimoreUSA

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